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Partially linearised plasmid DNA on untreated mica

The sample shown above is partially linearised plasmid DNA on untreated mica. The viewing area is 1 x 1 micron (256 x 256 pixels) and the movie is playing in real time (15 frames per second). These images were taken using the VideoAFM™ on a Veeco Bioscope.

Movie courtesey of N. Crampton and N. H. Thomson, University of Leeds, UK

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Imagine DNA .wmv movie | 920KB

Imagine DNA .avi movie | 4.11MB

Imagine DNA .mp4 movie | 729KB

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Screw dislocation

Real-time 2 µm x 2.25 µm movie of spiral growth forming from a screw dislocation in polyethylene oxide. A period of 70 ms lapses between each frame. This movie was collected using the VideoAFM™ in combination with a Veeco Dimension 3100.

Movie courtesy of J. K. Hobbs and C. Vasilev, University of Sheffield

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Screw Dislocation .wmv movie | 76KB

Screw Dislocation .avi movie | 4.62MB

Screw Dislocation .mp4 movie | 103KB

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Dendritic growth

VideoAFM movie showing the growth of a layer of polyethylene oxide confined at the surface after the main growth front has passed. Data collected at 14 frames/second. This movie was collected using the VideoAFM™ in combination with a Veeco Dimension 3100.

Movie courtesy of J. K. Hobbs and C. Vasilev, University of Sheffield

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Dendritic Growth .wmv movie | 1.78MB

Dendritic Growth .avi movie | 32.86MB

Dendritic Growth .mp4 movie | 423KB

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PHB crystallizing from the polymer melt

This movie was collected using the VideoAFM™ in combination with a Veeco Dimension 3100.

This movie shows the growth front of a polyhydroxybutyrate-co- valerate spherulite crystallizing from the melt. The smooth lower portion of the video is molten polymer. As the movie progresses, the crystallisation front gradually moves down the visible area until only crystallized material. A piece of dirt embedded in the melt can be seen in the lower right hand corner.

Each frame is made up from 256 x 256 pixels and was collected in 31 ms. The conventional AFM is not able to stably image the surface of the molten polymer when operating in contact mode. The surface of the crystal is topographically very smooth, with a total height contrast of approximately 10 nm and no sharp variations, hence the relatively low image contrast in these images. Towards the end of the movie the contrast was reduced a little as the piece of dirt referred to earlier is being pushed up by the motion of the melt as the growth front approaches, requiring the vertical scale to be extended. The movie is showing an area roughly 2.5µm x 2.5µm.

This movie highlights a very important difference and consequence of the high tip velocity of the VideoAFM compared to conventional AFM, allowing us to image much softer sample than would be expected.

Movie courtesey of L. Boszec, London Centre for Nanotechnolgy, UCL, London, UK

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Polymer Melt .wmv movie | 920KB

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Collagen fibres

The true video-rate of Infinitesima's VideoAFM™ enables interaction at the nanoscale. The above movie shows collagen fibres and demonstrates the flexibility of the system. Utilising the slow conventional AFM feedback to hop on and off the fibres (several hundreds of nanometres in height), the VideoAFM™ can maintain an image of the surface at 15 frames per second, using it's own integrated feedback. The movie clearly shows the 60 nm banding, characteristic to collagen. This movie was taken using a VideoAFM™ in combination with an NTMDT Smena.

Movie courtesey of L. Boszec, London Centre for Nanotechnolgy, UCL, London, UK

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Collagen Fibres .wmv movie | 310KB

Collagen Fibres .avi movie | 17.67MB

Collagen Fibres .mp4 movie | 192KB

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100 nm pitch Calibration Standard

The above movie shows the surface of a 100 nm pitch Calibration Standard. One half of the movie is at a scan size of about 2 microns and the other is at about 3 microns. This movie was taken using the VideoAFM™ in combination with a Veeco Multimode.

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100 nm pitch Calibration Standard .wmv movie | 357KB

100 nm pitch Calibration Standard .avi movie | 19.41MB

100 nm pitch Calibration Standard .mp4 movie | 204KB

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Collagen fibres in liquid

A single frame extracted from a movie, navigating around collagen fibres in a liquid environment. The image quite clearly shows the 67 nanometre banding on the fibres. The image was collected in 33 milliseconds. The VideoAFM™ was used in combination with an NTMDT Smena/Solver.

A segment from the movie can be downloaded below. The movie displays 256 x 256 pixels and was recorded in real-time at 15 frames per second.

Sample courtesy of Laurent Bozec, London Centre for Nanotechnology

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Liquid Environment .wmv movie | 547KB

Liquid Environment .avi movie | 18.26MB

Liquid Environment .mp4 movie | 306KB